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KMID : 1123920090230020464
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Korean Journal of Oriental Physiology and Pathology
2009 Volume.23 No. 2 p.464 ~ p.472
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Effects of Cheonghyul-San on the Generation of Redox Status and on the Expression of NF-B Dependent Proteins
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Oh Jeong-Pyo
Jung Ji-Chun
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Abstract
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The aim of this study was to investigate the effects of Cheonghyul-san on the generation of peroxynitrite (), nitric oxide (NO) and superoxide anion radical ( ), and on the expression of NF-B-dependent proinflammatory proteins in ob/ob mice. Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57BL/6J black mice) and control obese (ob/ob mice) groups have received the standard chow. The experimental groups were fed with a diet of chow supplemented with 7.5, 15 and 30 mg Cheonghyul-san per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein-2 (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blot was performed using anti-IKK-, anti-phospho IB-, anti-NF-B (p50, p65), anti-COX-2, anti-iNOS, anti-VCAM-1 antibodies, respectively. Cheonghyul-san prevented -induced cell death. Cheonghyul-san inhibited the generation of , NO and in the -treated LLC- cells. The generation of , NO and were inhibited in the Cheonghyul-san-administered ob/ob mice groups. The GSH/GSSG ratio was decreased in the ob/ob mice, whereas the ratio was improved in the Cheonghyul-san-administered groups. Cheonghyul-san inhibited the protein expression levels of phospho-IB-, IKK-, NF-B (p50, p65), COX-2, iNOS and VCAM-1 genes. These results suggest that Cheonghyul-san is an effective scavenger of , and NO, and has an inhibitory effect on the expression of NF-B-dependent inflammatory genes in ob/ob mice. Therefore, Cheonghyul-san might be used as a potential therapeutic drug against the diabetes- and obesity-related proinflammatory diseases.
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KEYWORD
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Cheonghyul-san, peroxynitrite, IKK-, IB-, NF-B, COX-2, iNOS
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